Thermo fisher scientific thermo scientific e1cliptip user manual 5 a. Components of the ettan ipgphor cup loading manifold system. For more information about the manifolds, see ettan ipgphor cup loading manifold user manual. Pdf proteomic analysis reveals the actin cytoskeleton as. After reduction and alkylation of disulfide bonds with 10 mgml dtt and 25 mgml iodoacetamide, respectively, the second.
Face the top of the thermometer toward you and place your thumb on the ridges. The ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, ease of use, speed, reproducibility, and high protein loading capacity ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold, ettan ipgphor manifold light, or ceramic strip holders. It485 6 1 h o tas manifold technical manual pengwyn. Equilibrated ipg strips were transferred onto 1 mm thick 25. This products resides on a fisher scientific gsa or va contract. Fifth avenue olum us, oh 43204 it48561hotas manifold technical manual 1st edition revision 1. Ettan ipgphor 3 with ettan ipgphor manifold offers a. Ettan ipgphor 3 control software user manual 11003459 ettan ipgphor 3 instrument user manual 11003458 ettan ipgphor cup loading manifold user manual 11003460 2d electrophoresis principles and methods 80642960 ettan dalttwelve system user manual 80647653.
Selective labelling of cellsurface proteins using cydye dige. Ettan ipgphor 3 isoelectric focusing unit 45002623 unit to run first dimension ief ipgphor manifold, complete 450026 for the most stringent applications. Proteome response at the edge of protein aggregation open. Electrophoresis, blotting and immunodetection electrophoresis equipment horizontal, ief 1d systems the improved, easytouse ettan ipgphor 3 system overcomes the most common hurdles of existing technology, to consistently deliver speed, reproducibility, and handle increased protein loads. If you are viewing this page as a nonregistered user, the prices displayed is list price. Instrument components five strip holder lengths are available 7, 11, 18, and 24 cmone for each ipg. Ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold, ettan ipgphor manifold light, or ceramic. Ettan ipgphor 3 operating instructions 28963551 ac 59 5 operation 5. We are here for you support download user manual or datasheet below.
Ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, speed, reproducibility and high protein loading capacity. Apply the cydyelabeled samples corresponding to 50 g total protein to immobiline drystrip gels by anodic cup loading in the manifold and perform isoelectric focusing ief using ettan ipgphor ii ief system according to instructions. The channel in the cup loading manifold is too wide to ensure proper absorption of the required volumes of rehydration solution. Ettan ipgphor 3 control software allows management of up to four ettan ipgphor 3 ief units simultaneously, each running a different set of run parameters. For detailed instructions on installation and usage of this software, refer to the ettan ipgphor 3 control software user manual. Ettan ipgphor ii isoelectric focusing system ipgphor ii function and description um 80650522 p2 control panel negative cathode electrode area positive anode electrode area ipgphor cup loading manifold ettan ipgphor ii control software computer not included cover pressure block sample application wells base 1. Firstdimension isoelectric focusing was conducted using the ettan ipgphor cup loading manifold ge healthcare and the following voltage ku0059436 price settings. Congenital anophthalmia and binocular neonatal enucleation.
The digital timer display the present time accurately. View online or download eaton 5px 3000i rt2u installation and user manual. The small tshaped protrusion fits into a cutout section of the ipgphor bed near the lid hinge fig 29. Ettan ipgphor 3 isoelectric focusing system, ge healthcare. Ge healthcare manifold kit for ettan ipgphor 3 isoelectric. Pdf visualization of proteins electrotransferred on hybond. Ettan ipgphor 3 isoelectric focusing unit 1 11003364 ipgphor manifold ceramic tray 1 80649857 electrode set 1 80649876 sample cups 120 80649895 ipgphor strip holder cleaning solution 950 ml 80645278 dalt gel 12.
The wholecell sample was dissolved in a solubilization solution 7 m urea, 2 m thiourea, 4% 33cholamidopropyl dimethylammonio1propanesulfonic acid chaps. Synchronized solutions for better 2d electrophoresis. Preparing ettan dalt electrophoresis units for electrophoresis using precast gels appendix i, extracted from 2d electrophoresis, ge healthcare, 2010 protocols for use of ettan daltsix and ettan dalttwelve differ in two main areas. Ettan ipgphor user manual 11003460 ad 2 description 2. Designed for maximum performance and flexibility, the manifolds can handle 12 ipg strips simultaneously. After rehydration, the strips were placed in an ettan ipgphor cup loading manifold ge healthcare. The newly designed manifold meets the evergrowing demands of proteomics research and delivers highresolution micropreparative protein analysis. Safety symbol general definitions of safety symbols used on the instrument or in manuals are listed below. Ettan ipgphor manifold, complete 80649838 ettan ipgphor manifold light, complete 11002688 ipgbox 28933465 ipgbox kit 28933492 immobiline drystrip cover fluid 173501 ipgphor manifold ceramic tray 80649857 ettan ipgphor manifold polymeric tray 11002580 immobiline drystrip gels, 24 cm immobiline drystrip ph 35. To improve understanding of the mechanisms of action and resistance to 2me2, we selected.
Ensure that the manifold is level by placing the round spirit level on the center of the manifold tray after it is placed on the ettan ipgphor. Plug the power cords into properly grounded outlets. Clean all parts with a sponge, hot water, and nonalkaline, non abrasive cleanser. Label the remaining 23 of the diseased sample with cy5. Use sample cups with ettan ipgphor manifold or ettan ipgphor manifold light to significantly increase protein loads. Crosslinking of dna repair and replication proteins to dna. Ettan ipgphor3 ief system 7cm strip protocols youtube. Proteomic analysis reveals the actin cytoskeleton as. Filter holder manifold user guide 11 how to clean the manifold immediately after use, clean the manifold parts to ensure optimum performance. More complex experimental designs can be generated using the standard. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3 isoelectric focusing system the ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, ease of use, speed, reproducibility, and high protein loading capacity. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3.
Isoelectric focusing was performed on an ettan ipgphor ief system using the ettan ipgphor cup loading manifold. Introduction congratulations on purchasing a thermo scientific e1cliptip electronic pipette. Ettan ipgphor isoelectric focusing system ipgphor function and description um 80641535 p2 control panel negative cathode electrode area positive anode electrode area cover pressure block sample application wells base 1. Sdspage in the second dimension was performed in 15% separation gels. The ettan ipgphor manifold is a highthroughput cup loading accessory for firstdimension isoelectric focusing of proteins on ipg strips. Ettan ipgphor 3 control software user manual 11003459 ettan ipgphor 3 instrument user manual 11003458 ettan ipgphor cup loading manifold user manual 11003460 2d electrophoresis principles and methods 80642960 ettan dalttwelve system user manual 80647653 ettan daltsix system user manual 80649249 typhoon user. Effects of various growth conditions in a chemostat on. Ettan ipgphor manifold is a accessory for ettan ipgphor ief unit allowing electrophoresis of large sample loads on ipg strip lengths from 724 cm. Ettan ipgphor 3 isoelectric focusing system also includes immobiline drystrip gel strips, which contain an immobilized ph gradient ipg. Ettan ipgphor 3 isoelectric focusing unit cytiva formerly. Isoelectric focusing was performed on an ettan ipgphor ief system using the ettan ipgphor cup loading manifold both ge healthcare. Lid design allows optimum running with lightsensitive protein samples.
Locate the battery compartment at the back of the thermometer. Ge healthcare ettan ipgphor strip holder cleaning solution. Run conditions were 300v for 3h, 600v for 3h, followed by a 6h gradient to v, a 3h gradient to 8000v and 8h at 8000v for a total of 4050kvh at 0. Pdf visualization of proteins electrotransferred on. Ettan ipgphor user manual 11003460 ad 7 2 description 2. Data file 28937442 aa 2d electrophoresis ipgbox and. Page 1 page 2 page 3 table of contents page 4 introduction page 5 environment page 6 installation page 7 page 8 unpacking and settingup the printer page 9 control panel page 10 printer states page 11 key functions page 12 loading material page inserting a media strip. Ipgphor manifold light 45002621 routine applications.
The thermo scientifictm e1cliptiptm is a general purpose electronic micropipette that operates on the airdisplacement principle. Isoelectric focusing ief was performed on an ettan ipgphor cup loading manifold system, according to manufacturers instructions. It is also compatible with the firstgeneration ettan ipgphor see ettan ipgphor user manual. Ipgphor strip holders various sizes ipgphor cup loading manifold. Press down firmly and slide the cover away from you, toward the bottom of the thermometer. Isoelectric focusing ief in the first dimension was carried out using an ettan ipgphor ii equipped with a cup loading manifold as a sample addition apparatus amersham biosciences.
Data file 28937442 aa 2d electrophoresis ipgbox and ipgbox kit. First dimension separation run on ettan ipgphor 3 ief unit, anodic cup loading. Cabinet mounting where the topway t2 manifold is installed in a metalbox manifold cabinet, install the thermostat in the enclosure above or to the side of the manifold assembly. Ief using ettan ipgphor 3 isoelectric focusing system and. Ensure that the manifold is level by placing the round spirit level on the center of the manifold tray after it is placed on the ettan ipgphor 3 unit. Tyco electronics te3124 operators manual pdf download. Cupbased sample application can improve proteinfocusing patterns, particularly in basic ipg strips, and the manifold accommodates either anodic or cathodic loading. Divided into compartments with a platform in the middle, the horizontal electrophoresis systems completely submerge plated gel platforms in a buffer. Ettan ipgphor 3 isoelectric focusing system function and description 1 ettan ipgphor 3 instrument user manual 11003458 edition ab 11 fig 14. Selective labelling of cellsurface proteins using cydye.
Cup based sample application can improve proteinfocusing patterns, particularly in basic ipg strips, and the manifold accommodates either anodic or cathodic loading. More complex experimental designs can be generated using the standard sample on all gels. Distributed io system system manual 082019 a5e31861578ag preface system overview 1 2 application planning 3 mounting 4 connecting 5 configuring 6 configuration control option handling 7 commissioning 8 maintenance 9 technical specifications 10 safetyrelated shutdown of et200al standard modules a dimension drawings b. Fluorescently labelled proteins were visualized using a typhoon 9400 variable mode imager and typhoon scanner control software, version 3. Do not use steel wool or abrasive materials since they can harm the components. Actual run conditions were 300 v for 3 h, 600 v for 3 h, followed by a 6h gradient to v, a 3h gradient to 8000 v, and 8. Selective labelling of cellsurface proteins using cydye dige fluor minimal dyes. Proteomic analysis reveals the actin cytoskeleton as cellular. Isoelectric focusing ief was performed on an ettan ipgphor cup loading manifold system according to manufacturers instructions. Ettan ipgphor manifold and manifold light simplify ief of multiple samples and reduce firstdimension handson time to a minimum. Heart samples from each animal was analyzed in quintuplicate by 2dge, i.
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